survey on o157:h7 enterohemorrhagic escherichia coli (ehec) in cattle in golestan province, iran.

نویسندگان

alireza koochakzadeh department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar-iran and department of microbiology, faculty of veterinary medicine, university of tehran, tehran-iran.

mahdi askari-badouei department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar-iran.

emad mazandarani department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar-iran.

omid madadgar department of microbiology, faculty of veterinary medicine, university of tehran, tehran-iran.

چکیده

background and objectives: a diverse group of escherichia coli are known as enterohemorrhagic escherichia coli (ehec) including o157:h7 and non-o157 ehec. enterohemorrhagic strains are related to severe clinical conditions in humans including hemorrhagic colitis and hemolytic uremic syndrome, and most of the recorded outbreaks occurred due to o157: h7 e. coli . the aim of the present study was to investigate the presence of o157:h7 e. coli among healthy cattle in golestan province. materials and methods: fecal samples were collected from 180 clinically healthy cattle in golestan province. after primary enrichment, samples were streaked on sorbitol macconkey agar supplemented with cefixime and potassium tellurite (ct-smac). non-sorbitol fermenting (nsf) escherichia coli isolates were subjected to serotyping using commercial o157 antisera and rf b o157 gene pcr. isolates were additionally tested for major virulence factors of ehec including stx1 , stx2 , eae and ehly by multiplex-pcr. results: eighteen nsf isolates were recovered from ct-smac confirmed as e. coli in biochemical tests. none of the obtained isolates belonged to o157 serogroup. overall, two isolates harbored the tested virulence genes; one isolate possessed stx2 and ehly, and the other one carried stx2 , eae and ehly . conclusion: the results of this study indicated that cattle in golestan province could be the reservoir for non-o157 ehec.

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Survey on O157:H7 enterohemorrhagic Escherichia coli (EHEC) in cattle in Golestan province, Iran

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عنوان ژورنال:
iranian journal of microbiology

جلد ۶، شماره ۴، صفحات ۲۷۶-۲۸۰

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